Planned Parenthood is suing Ohio's health department over a law designed to strip government money from the organization's affiliates in the state.
The funds, mostly federal, have supported HIV testing, promoted teen pregnancy prevention and provided help to new or expectant mothers.
Ohio's law is slated to take effect May 23. It would bar the money from going to entities that perform or promote abortions.
In a complaint filed Wednesday in federal court, Planned Parenthood says the law violates the organization's constitutional rights by denying it funding "in retaliation for" providing abortions. It wants the court to block the law from being enforced.
The organization has said the cuts would not force the closure of any of its 28 health centers in Ohio, but it would deprive patients of services.